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ATCC
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DSMZ
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CLS Cell Lines Service GmbH
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ImmunoTools
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STEMCELL Technologies Inc
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Promega
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Novo Nordisk
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BioResource International Inc
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Image Search Results
Journal: Biochemical pharmacology
Article Title: Tumor necrosis factor α-mediated inhibition of erythropoiesis involves GATA-1/GATA-2 balance impairment and PU.1 over-expression.
doi: 10.1016/j.bcp.2011.03.030
Figure Lengend Snippet: Fig. 3. Effect of TNFa on GATA-1 transcriptional activity (A) TF1 cells were co- transfected with the GATA-1-expressing vector, pXM-GATA1 and the reporter luciferase plasmids, pGL3-GATA-Luc and pHRG-TK. Transfected cells were treated for 2 h with TNFa or left untreated prior to analysis of luciferase activity. Firefly activity was normalized to Renilla, and the results are expressed as a ratio in arbitrary units. The data are presented as mean S.D. of three independent experiments (**P 0.01 and ***P < 0.001). (B) GATA-1 protein expression in TF1 cells cultured in the presence of GM-CSF (GM) or GM-CSF and TNFa (GM/TNF) was assessed by western blot analysis. Nuclear extracts from human erythroleukemia HEL cells were used as a positive control. b actin was used as an internal control.
Article Snippet:
Techniques: Activity Assay, Transfection, Expressing, Plasmid Preparation, Luciferase, Cell Culture, Western Blot, Positive Control, Control